#Realtimechem week is only 8 days away! As @realtimechem/@doctor_galactic has mentioned, it is hoped that people will blog as much as possible during #realtimechem week. Each evening, or as often as I can, I will collate all the posts here. It would be great if you could either tweet me (@jessthechemist), email me (theorganicsolutionblog at gmail dot com) or simply tweet the link but remember to use the hashtag #realtimechemcarnival so that I can find it easily.
As an indicator, I thought I would show you what I plan on sharing during #realtimechemcarnival. These photos are from an afternoon I spent in the lab last week and demonstrate a typical afternoon in my lab.
We are lucky enough to have an automated column in my lab which makes my life so much easier as I don’t have to column by hand. Firstly I TLC to see what solvent conditions are best and then I get started with the column. Automated columns are great as you can change the solvent gradient with a click of a button. In this particular case, my product was a very polar, “sticky” amine (the spot pretty much on the baseline) which meant that once the small by-products came off the column, I could increase the polarity of the solvents to get the awkward product off.
As you can see, the column resulted in me having a lot of test tubes to deal with. The column gives me a UV trace which tells me which test tubes have my product in but I am a little anal so I still TLC the test tubes.
After I have confirmed where my product is by TLC, I pour all the solvent from the relevant test tubes into a round bottomed flask and get vacc’ing off the solvent on the rotary evaporator.
After all that work, I end up with a product which I can analyse by NMR and mass spectroscopy (GC-MS in this case). As you can see, the GC trace shows only one product which was confirmed as my desired compound by mass spec.
After all of this, it is time for washing up.