#Realtimechem Carnival

#Realtimechem week is only 8 days away! As @realtimechem/@doctor_galactic has mentioned, it is hoped that people will blog as much as possible during #realtimechem week. Each evening, or as often as I can, I will collate all the posts here. It would be great if you could either tweet me (@jessthechemist), email me (theorganicsolutionblog at gmail dot com) or simply tweet the link but remember to use the hashtag #realtimechemcarnival so that I can find it easily.

As an indicator, I thought I would show you what I plan on sharing during #realtimechemcarnival. These photos are from an afternoon I spent in the lab last week and demonstrate a typical afternoon in my lab.

We are lucky enough to have an automated column in my lab which makes my life so much easier as I don’t have to column by hand. Firstly I TLC to see what solvent conditions are best and then I get started with the column. Automated columns are great as you can change the solvent gradient with a click of a button.  In this particular case, my product was a very polar, “sticky” amine (the spot pretty much on the baseline) which meant that once the small by-products came off the column, I could increase the polarity of the solvents to get the awkward product off.

 TLC-1Column-1

As you can see, the column resulted in me having a lot of test tubes to deal with. The column gives me a UV trace which tells me which test tubes have my product in but I am a little anal so I still TLC the test tubes.

tubes-2tubes1

After I have confirmed where my product is by TLC, I pour all the solvent from the relevant test tubes into a round bottomed flask and get vacc’ing off the solvent on the rotary evaporator.

tubes-3buchi1

After all that work, I end up with a product which I can analyse by NMR and mass spectroscopy (GC-MS in this case). As you can see, the GC trace shows only one product which was confirmed as my desired compound by mass spec.

product-1GCMS-1

After all of this, it is time for washing up.

Advertisements

12 thoughts on “#Realtimechem Carnival

  1. Pingback: Real Time Chem Week: The FAQ (updated!) | Doctor Galactic & The Lab Coat Cowboy

  2. We chemist should start using analytic or analytical… Anal sound just weird, too weird (coff coff Sigma-Aldrich FixAnal)
    from your post: “but I am a little anal so I still TLC the test tubes.” 🙂

  3. Automatic column chromatography machine?!?! WOW! I would like me one of those, please! I also wish we had easy access to GC-MS in the lab that I’m in. Sigh.

    What are the things in the water bath of the rotavap?

    While every week is pretty much #realtimechem week for me, I’ll be sure to put some extra effort into #realtimechemcarnival. I’ll also make sure I’ve got my blog-head-on for next week! I have been somewhat slack lately.

    • Yes, we are very lucky in my lab, we have 2 x GC, one GCMS, on DSC, auto column and a HPLC. Other people can use our equipment too but it is nice having quick access.

      The balls keep the heat in the water bath so it doesn’t evaporate as quickly. A lot of people have asked that question, are they not common in the rest of the world?

      • Never seen the balls before… But my lab experience is limited to the School of Chemistry at Sydney University. The big organic lab (that I’m in) has a few of LCMSs and HPLCs. I guess we’re very lucky in that sense as well, except I don’t really use that equipment.

        Unfortunate that we don’t have a decent GC. They’re so much faster! Great for small molecules. I really miss the nice one that I used when I was working in the inorganic lab.

  4. Nice blog! I will surely visit again! btw -mass spectrometry- is the correct name, but surely you already knew that as you link to the right name/page on wikipedia =D

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s